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Cyclooxygenase‐2 up‐regulation after FLAP transfection in human adenocarcinoma cell line HT29 cl.19A
Author(s) -
Battu Serge,
Beneytout Jean Louis,
Pairet Michel,
Rigaud Michel
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01196-x
Subject(s) - transfection , cyclooxygenase , cell culture , chemistry , adenocarcinoma , cancer research , line (geometry) , microbiology and biotechnology , medicine , biology , biochemistry , cancer , enzyme , genetics , geometry , mathematics
Five‐lipoxygenase‐activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5‐lipoxygenase pathway. In the enterocyte model HT29 cl.19 A cell line, 5‐lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents an original mammalian model to study FLAP‐dependent leukotriene synthesis. In FLAP cDNA transfected HT29 cl.19 A cells, FLAP expression led to an increase in cyclooxygenase pathway products (mainly PGE 2 ) without an increase in 5‐lipoxygenase metabolism. This increase in PGE 2 synthesis was associated with a cyclooxygenase‐2 up‐regulation in comparison to untransfected HT29 cl.19A cells. These results suggest a possible interaction between the two major pathways of arachidonic acid metabolism.