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Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron‐responsive element
Author(s) -
Lind Maria I.,
Ekengren Sophia,
Melefors Öjar,
Söderhäll Kenneth
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01186-7
Subject(s) - ferritin , polyadenylation , alternative splicing , rna splicing , rna , messenger rna , biology , drosophila melanogaster , microbiology and biotechnology , protein subunit , untranslated region , three prime untranslated region , gene , biochemistry
Several mRNAs encoding the same ferritin subunit of Drosophila melanogaster were identified. Alternative RNA splicing and utilisation of different polyadenylation sites were found to generate the transcripts. The alternative RNA splicing results in ferritin transcripts with four unique 5′ untranslated regions. Only one of them contains an iron‐responsive element. The iron‐responsive element was found to bind in vitro specifically to human recombinant iron regulatory protein 1. Furthermore, the ferritin subunit mRNAs are differentially expressed during development. Our data provides the first molecular evidence that the presence of iron‐responsive element in a ferritin mRNA is regulated by alternative RNA splicing.