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GATA‐3 represses gp91 phox gene expression in eosinophil‐committed HL‐60‐C15 cells
Author(s) -
Anowar Sadat Mohammed,
Kumatori Atsushi,
Suzuki Shoichi,
Yamaguchi Yuji,
Tsuji Yoshiro,
Nakamura Michio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01182-x
Subject(s) - microbiology and biotechnology , electrophoretic mobility shift assay , transfection , gene , promoter , gene expression , binding site , mutation , biology , chemistry , genetics
To study the regulatory mechanism of gp91 phox gene expression in eosinophils, we transiently transfected eosinophil‐committed HL‐60‐C15 cells with gp91 phox promoter constructs, and identified a negative element from bp −267 to −246 of the gp91 phox gene, the deletion of which caused an 83% increase in promoter activity. Electrophoresis mobility shift assays demonstrated GATA‐3 binds to the GATA consensus site from bp −256 to −250. An 81% increment in promoter activity was obtained when a mutation was introduced in the GATA‐3 binding site of the bp −267 to +12 construct, which is comparable to that of the bp −245 to +12 construct. We therefore conclude that GATA‐3 specifically binding to the GATA site negatively regulates the expression of the gene in HL‐60‐C15 cells.