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Impaired production of nitric oxide, superoxide, and hydrogen peroxide in glucose 6‐phosphate‐dehydrogenase‐deficient granulocytes
Author(s) -
Tsai Kan-Jen,
Hung Iou-Jih,
Chow Ching Kwong,
Stern Arnold,
Chao Sin Sin,
Chiu Daniel Tsun-Yee
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01174-0
Subject(s) - superoxide , nadph oxidase , nitric oxide , chemistry , hydrogen peroxide , biochemistry , nitric oxide synthase , granulocyte , dehydrogenase , pentose phosphate pathway , glucose 6 phosphate dehydrogenase , reactive oxygen species , lipopolysaccharide , nitrite , oxidase test , microbiology and biotechnology , endocrinology , medicine , enzyme , biology , immunology , glycolysis , organic chemistry , nitrate
Since the generation of superoxide and hydrogen peroxide by NADPH oxidase and nitric oxide (NO) by NO synthase (NOS) in granulocytes is NADPH‐dependent, we investigated the production of NO, superoxide and H 2 O 2 in glucose 6‐phosphate dehydrogenase (G6PD)‐deficient human granulocytes. Our results showed that upon stimulation with either 5 μg/ml of lipopolysaccharide (LPS) or 10 μM of phorbol 12‐myristate 13‐acetate (PMA), the production of nitrite in normal granulocytes was elevated, 252±135% and 239±72%, respectively, compared to the resting stage. In contrast, G6PD‐deficient granulocytes did not produce more nitrite upon stimulation with either LPS or PMA compared to the resting stage. Western blot analysis indicated a normal expression pattern of inducible NOS in G6PD‐deficient granulocytes. In addition, the production of H 2 O 2 and superoxide was also significantly impaired in G6PD‐deficient granulocytes compared to control cells. These data demonstrate that G6PD deficiency causes an impairment in the production of NO, superoxide and H 2 O 2 .