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Characterization of a novel structural member, LukE‐LukD, of the bi‐component staphylococcal leucotoxins family
Author(s) -
Gravet A.,
Colin D.A.,
Keller D.,
Giradot R.,
Monteil H.,
Prévost G.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01130-2
Subject(s) - staphylococcus aureus , immunoprecipitation , family member , microbiology and biotechnology , biology , antibody , protein a , staphylococcus , strain (injury) , gene , chemistry , bacteria , genetics , medicine , family medicine , anatomy
A new member of the staphylococcal bi‐component leucotoxins family, LukE (32 kDa) and LukD (34.3 kDa) has been characterized from Staphylococcus aureus strain Newman. LukE was 58–68% identical with the class S proteins, whereas LukD was 71–77% identical with the class F proteins of the family. A partial immunoreactivity with the various affinity‐purified antibodies specific for the other proteins was observed. Immunoprecipitation assay and gene probing confirmed a 30% frequency among human clinical isolates, differing from the distribution of the other known leucotoxins ( P <0.005). LukE+LukD was as effective as the Panton‐Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus .