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Matrilin‐4, a new member of the matrilin family of extracellular matrix proteins 1
Author(s) -
Wagener Raimund,
Kobbe Birgit,
Paulsson Mats
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01111-9
Subject(s) - complementary dna , signal peptide , amino acid , messenger rna , peptide sequence , coding region , extracellular matrix , biology , microbiology and biotechnology , chemistry , biochemistry , gene
Mouse cDNA encoding for matrilin‐4 was cloned and the primary structure of this fourth member of the matrilin family was deduced from the nucleotide sequence. The protein precursor of 624 amino acids consists of a putative signal peptide, two vWFA‐like domains linked by four epidermal growth factor‐like modules and a potential coiled‐coil α‐helical oligomerization domain at the C‐terminus. The predicted M r of the mature protein is 66 442. Expression in lung, brain, sternum, kidney and heart was detected by Northern blot analysis of mouse mRNA. Additionally an alternatively spliced mRNA lacking the sequence coding for the first vWFA domain was found in 7 weeks old mice leading to a protein precursor of 434 amino acids and a predicted M r of the mature protein of 45 468.