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Enhanced fMLP‐stimulated chemotaxis in human neutrophils from individuals carrying the G protein β3 subunit 825 T‐allele
Author(s) -
Virchow Sebastian,
Ansorge Nikolaus,
Rübben Herbert,
Siffert Gerlinde,
Siffert Winfried
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01110-7
Subject(s) - heterotrimeric g protein , pertussis toxin , chemotaxis , g protein , allele , genotype , microbiology and biotechnology , gi alpha subunit , protein subunit , signal transduction , chemokine , biology , g alpha subunit , gene , chemistry , immune system , immunology , receptor , biochemistry
We have recently described a C825T polymorphism in the gene encoding for the Gβ3 subunit of heterotrimeric G proteins. The 825T allele is associated with a novel splice variant (Gβ3‐s) and enhanced signal transduction via pertussis toxin (PTX)‐sensitive G proteins. fMLP‐induced chemotaxis, but not O 2− generation, was increased in neutrophils with the TC/TT (EC 50 =1.5±1.3 nM) genotypes compared to the CC genotype (EC 50 =5.9±1.5 nM). Maximal fMLP‐induced increase in [Ca 2+ ] i was significantly reduced in neutrophils from individuals with TC/TT genotype vs. CC genotype (212.9±10.1 nM vs. 146.4±24.2 nM). Gβ3‐s appears to be associated with enhanced immune cell function in humans.