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Up‐regulation of the expression of major histocompatibility complex class I antigens by plasmid DNA transfection in non‐hematopoietic cells
Author(s) -
Park Joo-Hung,
Chang Sun-Hee,
Kim Min-Chul,
Shin Sung-Hye,
Youn Hyun-Joo,
Kim Jin-Kyoo,
Jang Yong-Suk,
Kim Chul-Woo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01097-7
Subject(s) - transfection , microbiology and biotechnology , major histocompatibility complex , mhc class i , biology , electroporation , transporter associated with antigen processing , mhc restriction , cd74 , northern blot , plasmid , dna , antigen , gene , gene expression , genetics
The effect of DNA on the surface expression of major histocompatibility (MHC) class I antigens was examined in non‐hematopoietic tumor cell lines. Transfection with plasmid DNA via liposome or electroporation significantly increased the surface expression of MHC class I molecules in a transient manner. Northern blot analysis showed that levels of MHC class I mRNA were increased by DNA transfection, probably via transcriptional activation. In contrast, the expression of the MHC class II and β‐actin genes was not affected, suggesting that the up‐regulation of MHC class I expression by plasmid DNA works in a gene‐specific manner.