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Telomerase from Saccharomyces cerevisiae contains several protein subunits and may have different activities depending on the protein content
Author(s) -
Petrov Andrey V,
Dokudovskaya Svetlana S,
Sokolov Kirill A,
Lavrik Olga I,
Favre Alain,
Dontsova Olga A,
Bogdanov Alexey A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01091-6
Subject(s) - telomerase , telomere , saccharomyces cerevisiae , ribonucleoprotein , processivity , telomerase rna component , protein subunit , nuclease , biochemistry , biology , microbiology and biotechnology , chemistry , yeast , dna , dna replication , rna , telomerase reverse transcriptase , gene
Telomerase is a ribonucleoprotein responsible for maintaining telomeres during the cell cycle [1, 2]. Here we describe a two‐step purification procedure for the Saccharomyces cerevisiae telomerase complex. We have found that the properties (processivity, nuclease activity) of telomerase depend on the isolation procedure. Using a cross‐linking approach, we have revealed several proteins that could be components of the telomerase complex. Furthermore, spectra of cross‐linked proteins differ in processive and non‐processive telomerase complexes.