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NSF is required for the brefeldin A‐promoted disassembly of the Golgi apparatus
Author(s) -
Fukunaga Takuya,
Furuno Akiko,
Hatsuzawa Kiyotaka,
Tani Katsuko,
Yamamoto Akitsugu,
Tagaya Mitsuo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01081-3
Subject(s) - brefeldin a , golgi apparatus , copi , microinjection , microbiology and biotechnology , chemistry , golgi membrane , secretory pathway , protein subunit , vesicle , biology , biochemistry , endoplasmic reticulum , membrane , gene
N ‐Ethylmaleimide‐sensitive factor (NSF) is required for multiple pathways of vesicle‐mediated protein transport. Microinjection of a monoclonal anti‐NSF antibody almost completely blocked brefeldin A‐promoted Golgi disassembly without affecting the rapid release of β‐COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant‐negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF‐mediated step is present in the brefeldin A‐promoted disassembly of the Golgi apparatus.