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The effects of SNAP/SNARE complexes on the ATPase of NSF
Author(s) -
Matveeva Elena,
Whiteheart Sidney W.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01071-0
Subject(s) - snare complex , synaptobrevin , chemistry , snap , syntaxin , atpase , biophysics , vesicle , stimulation , lipid bilayer fusion , atp hydrolysis , microbiology and biotechnology , biochemistry , synaptic vesicle , biology , membrane , enzyme , neuroscience , computer graphics (images) , computer science
The ATPase of the N ‐ethylmaleimide sensitive factor (NSF) appears to be central to the events that culminate in vesicle‐target membrane fusion. Complexes containing different combinations of NSF, α‐SNAP, Vamp‐2 (synaptobrevin 2), syntaxin 1, and SNAP‐25 were reconstituted and then tested for their effect on the ATPase of NSF. While NSF interacts with all α‐SNAP‐containing complexes, only the α‐SNAP/t‐SNARE complex significantly stimulated ATPase activity. This stimulation was dependent on increasing SNAP/t‐SNARE complex and was saturable. The apparent stimulation of ATPase activity is due to a 10‐fold increase in initial hydrolysis rate. Complex containing both v‐ and t‐SNAREs bound significantly more α‐SNAP but did not stimulate the ATPase of NSF.