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Novel defensin subfamily from spinach ( Spinacia oleracea )
Author(s) -
Segura Ana,
Moreno Manuel,
Molina Antonio,
Garcı́a-Olmedo Francisco
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01060-6
Subject(s) - spinacia , spinach , biology , microbiology and biotechnology , clavibacter michiganensis , fusarium solani , antimicrobial peptides , subfamily , antimicrobial , botany , hypha , biochemistry , chloroplast , pathogen , gene
Antimicrobial peptides (So‐D1‐7) were isolated from a crude cell wall preparation from spinach leaves ( Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So‐D2‐7) represented a novel structural subfamily of plant defensins (group IV). Group‐IV defensins were also functionally distinct from those of groups I–III. They were active at concentrations <20 μM against Gram‐positive ( Clavibacter michiganensis ) and Gram‐negative ( Ralstonia solanacearum ) bacterial pathogens, as well as against fungi, such as Fusarium culmorum , F. solani , Bipolaris maydis , and Colletotrichum lagenarium . Fungal inhibition occurred without hyphal branching. Group‐IV defensins were preferentially distributed in the epidermal cell layer of leaves and in the subepidermal region of stems.