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Growth factor protection against cytokine‐induced apoptosis in neonatal rat islets of Langerhans: role of Fas
Author(s) -
Harrison M.,
Dunger A.M.,
Berg S.,
Mabley J.,
John N.,
Green M.H.L.,
Green I.C.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01051-5
Subject(s) - apoptosis , cytokine , islet , cytolysis , tumor necrosis factor alpha , programmed cell death , fas receptor , endocrinology , antibody , necrosis , interferon gamma , growth factor , insulin , biology , immunology , medicine , chemistry , biochemistry , receptor , cytotoxicity , in vitro
Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin‐1β 10 −10 M, tumour necrosis factor‐α 10 −10 M, interferon‐γ 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti‐Fas cytolytic antibody JO2. Pre‐treatment with insulin (25 ng/ml) or insulin‐like growth factor‐1 (10 −8 M) gave only partial protection against cell killing, but prevented the Fas‐mediated component. In the absence of cytokine treatment, Fas‐mediated killing was not observed.