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Gos1p, a Saccharomyces cerevisiae SNARE protein involved in Golgi transport
Author(s) -
McNew James A.,
Coe John G.S.,
Søgaard Morten,
Zemelman Boris V.,
Wimmer Christian,
Hong Wanjin,
Söllner Thomas H.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01044-8
Subject(s) - golgi apparatus , saccharomyces cerevisiae , microbiology and biotechnology , secretory pathway , vesicular transport protein , transport protein , biology , vesicular transport proteins , secretory protein , secretion , protein targeting , yeast , membrane protein , chemistry , biochemistry , endoplasmic reticulum , vacuolar protein sorting , membrane , vesicle
Specific transport between secretory compartments requires that vesicular carriers contain targeting proteins known as SNAREs. Ten v‐SNAREs have been identified in the genome of the yeast Saccharomyces cerevisiae by sequence analysis. We report here the characterization of Gos1p, a v‐SNARE localized to the Golgi compartment and likely homolog of the mammalian protein GOS‐28/GS28. Gos1p is a type II membrane protein with characteristic SNARE sequence hallmarks and is functionally a SNARE protein. Gos1p was originally identified as a 28 kDa protein in an immunoprecipitate of the cis ‐Golgi t‐SNARE Sed5p. This interaction between Sed5p and Gos1p is direct as demonstrated by in vitro binding with recombinant proteins. Deletion of GOS1 results in viable haploids with modest growth and secretory defects. Close examination of the secretory phenotype of GOS1 ‐disrupted cells suggests that Gos1p may play a role in multiple transport steps, specifically ER‐Golgi and/or intra‐Golgi transport.