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Anisoosmotic regulation of the Mi‐2 autoantigen mRNA in H4IIE rat hepatoma cells and primary hepatocytes
Author(s) -
Hammermann Rainer,
Warskulat Ulrich,
Häussinger Dieter
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01030-8
Subject(s) - messenger rna , primary (astronomy) , chemistry , microbiology and biotechnology , biology , biochemistry , gene , physics , astronomy
Using the differential display polymerase chain reaction (DDRT‐PCR) a 169 bp cDNA product, which is 88.8% homologous to the human Mi‐2β autoantigen, was identified in H4IIE rat hepatoma cells. At protein level 100% homology was found. The Mi‐2 mRNA was downregulated after hypoosmotic exposure and upregulated after hyperosmotic exposure in H4IIE cells and rat hepatocytes. The human Mi‐2 is an autoantigen in dermatomyositis and is a member of the SNF/RAD 54 helicase family. Accordingly, Mi‐2 may not only be a target of osmosignalling but could also be involved in the osmosignalling pathway towards gene expression in H4IIE and liver parenchymal cells.