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Isolation and characterization of a rice cDNA encoding the γ‐subunit of translation elongation factor 1B (eEF1Bγ) 1
Author(s) -
Kidou Shin-ichiro,
Tsukamoto Shigenori,
Kobayashi Satoru,
Ejiri Shin-ichiro
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01014-x
Subject(s) - elongation factor , complementary dna , open reading frame , biology , microbiology and biotechnology , protein subunit , southern blot , eukaryotic translation elongation factor 1 alpha 1 , gene , genetics , peptide sequence , rna , ribosome
We isolated a rice cDNA clone ( refg ) encoding the γ‐subunit of translation elongation factor 1B (eEF‐1Bγ; the old designation was EF‐1γ). The refg encodes an open reading frame of 419 amino acids which shows a similarity to the equivalent sequences from animals and yeast. Complex formation analysis, which showed the recombinant protein of refg (His‐eEF1Bγ) and formed a complex with GST‐eEF‐1Bβ, indicated that the refg encodes rice eEF1Bγ of the eEF1Bαβγ complex. Expression analysis showed that refg mRNA is very abundant in suspension‐cultured cells during the exponential phase of growth. A DNA blot analysis indicated that refg is located at a single locus in the rice genome.