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Observing the deoxy myoglobin and hemogobin signals from rat myocardium in situ
Author(s) -
Tran Tuan-Khanh,
Kreutzer Ulrike,
Jue Thomas
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)01001-1
Subject(s) - myoglobin , hemoglobin , in situ , chemistry , in vivo , globin , nuclear magnetic resonance , biophysics , microbiology and biotechnology , biochemistry , biology , physics , organic chemistry
1 H NMR proximal histidyl N δ H signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO 2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O 2 , the erythrocyte Hb is partially desaturated and yields the α and β proximal histidyl N δ H signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO 2 .