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On the expression of cytosolic calcium‐independent phospholipase A 2 (88 kDa) in immature and mature myeloid cells and its role in leukotriene synthesis in human granulocytes
Author(s) -
Larsson Forsell Pontus K.A.,
Runarsson Gudmundur,
Ibrahim Mirna,
Björkholm Magnus,
Claesson Hans-Erik
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00999-5
Subject(s) - zymosan , arachidonic acid , cytosol , phospholipase a , phospholipase a2 , leukotriene , arachidonate 5 lipoxygenase , phospholipase , calcium , biology , ionophore , granulocyte , microbiology and biotechnology , monocyte , chemistry , biochemistry , immunology , enzyme , in vitro , organic chemistry , asthma
The human calcium‐independent phospholipase A 2 (iPLA 2 ; 88 kDa) has recently been cloned (Larsson, P.K.A., Claesson, H.‐E. and Kennedy, B.P. (1998) J. Biol. Chem. 272, 207–214). Here we demonstrate the expression of the human iPLA 2 mRNA and its splice variants in blood progenitor cells, immature leukemic cells and mature granulocytes. Chromatographical resolvable iPLA 2 activity was found in the cytosolic fraction of granulocytes and the activity was inhibited by the iPLA 2 inhibitor bromoenol lactone. This drug also inhibited leukotriene synthesis in human granulocytes, induced by low concentration of calcium ionophore A23187 (0.10–0.15 μM) or opsonized zymosan. These results suggest that iPLA 2 is involved in the regulation of the pool of arachidonic acid destined for leukotriene synthesis in human granulocytes.