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A new human nm23 homologue ( nm23 ‐H5) specifically expressed in testis germinal cells 1
Author(s) -
Munier Annie,
Feral Chloé,
Milon Laurence,
Pi Véronique Phung-Ba,
Gyapay Gabor,
Capeau Jacqueline,
Guellaen Georges,
Lacombe Marie-Lise
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00996-x
Subject(s) - microbiology and biotechnology , chemistry , biology , physics
We have identified a cDNA encoding a 212 amino acid protein (Nm23‐H5) with 27–31% identity to the human members of the nm23 /nucleoside diphosphate (NDP) kinase gene family. The nm23 ‐H5 gene is located on chromosome 5q23–31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed in testis, in the spermatogonia and early spermatocytes. Nm23‐H5 possesses most of the residues conserved among NDP kinases plus an additional COOH‐terminus end of 55 amino acids unique to this protein. However, under usual assay conditions, Nm23‐H5 expressed in Escherichia coli did not exhibit NDP kinase activity. These results suggest that Nm23‐H5 is specifically involved in early stages of spermatogenesis.