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Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy
Author(s) -
Okada Takashi,
Swoboda Ines,
Bhalla Prem L,
Toriyama Kinya,
Singh Mohan B
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00992-2
Subject(s) - hypoallergenic , mutant , allergen , brassica , pollen , immunotherapy , chemistry , biology , biochemistry , microbiology and biotechnology , allergy , immunology , botany , immune system , gene
The Brassica pollen allergen Bra r 1 belongs to a new family of Ca 2+ ‐binding proteins, characterized by the presence of two potential EF‐hand calcium‐binding domains. Disruption of these EF‐hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca 2+ ‐binding sites. Calcium‐binding deficient mutants displayed significantly reduced IgE‐binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy.