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pH‐sensitive liposomes for receptor‐mediated delivery to chicken hepatoma (LMH) cells
Author(s) -
Škalko Nataša,
Peschka Regine,
Altenschmidt Uwe,
Lung Andreas,
Schubert Rolf
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00984-3
Subject(s) - liposome , asialoglycoprotein receptor , bovine serum albumin , chemistry , dextran , biophysics , chromatography , receptor , confocal microscopy , biochemistry , in vitro , microbiology and biotechnology , biology , hepatocyte
pH‐sensitive liposomes composed of dioleoylphosphatidylethanolamine and cholesterol hemisuccinate (3:2 mol/mol) bearing the N ‐acetylglucosamine derivative of bovine serum albumin (N‐Ac‐BSA) were applied for receptor‐mediated delivery in chicken hepatoma (LMH) cells expressing the N‐Ac‐BSA‐binding asialoglycoprotein receptor. Fluorescently labeled dextran was entrapped in liposomes by a modified freeze‐thawing method (encapsulation efficiency of 23%). A novel method of coupling proteins onto the surface of preformed liposomes yielded a coupling efficiency of 60–70%. The association of pH‐sensitive and lecithin liposomes with LMH cells was monitored by fluorescence‐activated cell sorting and confocal microscopy. Prerequisites for receptor‐mediated delivery to LMH cells were both the pH sensitivity of liposomes and the presence of N‐Ac‐BSA on the liposomal surface.