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Characterisation of high M r wheat glutenin polymers by agarose gel eletrophoresis and dynamic light scattering
Author(s) -
Egorov T.A.,
Odintsova T.I.,
Shewry P.R.,
Tatham A.S.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00983-1
Subject(s) - glutenin , agarose , polymer , dynamic light scattering , light scattering , chromatography , electrophoresis , wheat gluten , chemistry , materials science , gluten , scattering , analytical chemistry (journal) , biochemistry , organic chemistry , optics , nanotechnology , physics , protein subunit , nanoparticle , gene
Agarose gel electrophoresis has been used to separate the complex mixture of wheat gluten polymers into fractions ranging in M r , determined by dynamic light scattering, from about 500 000 to over 5×10 6 . The separation is reliable and reproducible and well suited to the routine analysis of multiple samples.