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Superoxide radical production by sponges Sycon sp
Author(s) -
Peskin Alexander V,
Labas Yulii A,
Tikhonov Alexander N
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00981-8
Subject(s) - tiron , sponge , catechol , superoxide , scavenger , chemistry , superoxide dismutase , radical , superoxide radical , seawater , biochemistry , botany , biology , antioxidant , ecology , enzyme
Using the catechol Tiron as an O −⋅ 2 scavenger, we showed that sea sponges ( Sycon sp.) produce superoxide radicals in sea water at a high rate without any stimuli added. The rate of O −⋅ 2 outflow from sponges to their water surroundings reaches a value of 0.5 nmol/min per sponge at pH 6.5. The generation of O −⋅ 2 was inhibited by Cu,Zn‐superoxide dismutase, and restored by the addition of KCN. We also confirmed the abiotic production of O −⋅ 2 in sea water, detected earlier with a different method by Petasne and Zika [Nature 325 (1987) 516–518].