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Possible stage‐specific function of NF‐κB during pre‐B cell differentiation
Author(s) -
Guerrini Luisa,
Molteni Angela,
Blasi Francesco
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00965-x
Subject(s) - nf κb , transcription factor , microbiology and biotechnology , transcription (linguistics) , cellular differentiation , b cell , gene , lipopolysaccharide , cell culture , chemistry , nfkb1 , iκbα , gene expression , biology , phenotype , cell , nucleus , immunology , biochemistry , antibody , signal transduction , genetics , linguistics , philosophy
Lipopolysaccharide (LPS)‐induced differentiation of the murine pre‐B cell line 70Z/3 is a model for pre‐B to B cell differentiation and has been used to show that the transcription factor NF‐κB is essential to induce the expression of the Igκ gene. We have investigated the mechanism involved in late stages of the process when all cells have reached a more mature B phenotype, i.e. beyond 48 up to 96 h of LPS treatment. NF‐κB binding activity was induced at early times by LPS treatment, but its DNA binding activity disappeared after 84 h of LPS treatment. Accumulation of IκBα protein in the nucleus correlated with the disappearance of NF‐κB activity at 72, 84 and 96 h, and treatment of nuclear extracts of 72–96 h LPS‐treated cells with Na‐deoxycholate restored NF‐κB binding activity. The data indicate that NF‐κB, while important to initiate the process of Igκ gene transcription in 70Z/3 pre‐B cells, is no longer required for its maintenance in differentiated 70Z/3 cells.