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Promoter‐independent regulation of cell‐specific dopamine receptor expression
Author(s) -
Knapp Mark,
Wong Albert H.C,
Schoots Oscar,
Guan H.C,
Van Tol Hubert H.M
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00961-2
Subject(s) - heterologous , receptor , biology , heterologous expression , transcription factor , dopamine , microbiology and biotechnology , cell , gene expression , transcription (linguistics) , cell culture , in vitro , dopamine receptor , receptor expression , gene , recombinant dna , genetics , endocrinology , linguistics , philosophy
Here we describe the construction of recombinant adenoviruses expressing dopamine D2 and D4 receptors, and their ability to mediate high levels of heterologous expression in a variety of cell types in vitro and in vivo for at least 7 days post infection. These experiments demonstrated that maximum receptor expression is achieved generally within 24 h and remains constant thereafter. Maximum expression levels were highly variable between cell lines and dependent on infection efficiency and promoter strength. Correction for these two variables revealed differences in relative expression levels between cell lines varying by two orders of magnitude. Our results indicate that in addition to gene transcription, post‐transcriptional mechanisms play a dominant role in determining dopamine receptor levels in this system.