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Modification of pigment composition in the isolated reaction center of photosystem II
Author(s) -
Gall Bernhard,
Zehetner Andrea,
Scherz Avigdor,
Scheer Hugo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00956-9
Subject(s) - photosynthetic reaction centre , photosystem ii , pigment , light harvesting complexes of green plants , chemistry , photochemistry , chlorophyll , circular dichroism , photosystem i , chlorophyll a , absorption (acoustics) , fluorescence , p700 , photosynthetic pigment , absorption spectroscopy , photosystem , photosynthesis , stereochemistry , biochemistry , materials science , organic chemistry , electron transfer , optics , physics , composite material
The pigment content of isolated reaction centers of photosystem II was modified using an exchange protocol similar to that used for purple bacterial reaction centers. With this method, which is based on incubation of reaction centers at elevated temperature with an excess of chemically modified pigments, it was possible to incorporate [3‐acetyl]‐chlorophyll a and [Zn]‐chlorophyll a into photosystem II reaction centers. Pigment exchange has been verified by absorption, circular dichroism and fluorescence spectroscopy, and quantitated by HPLC analysis of pigment extracts.