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Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus
Author(s) -
Russo Silvia,
Montermini Laura,
Berkovitz-Siman-Tov Revital,
Ponti Wilma,
Poli Giorgio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00951-x
Subject(s) - recombinant dna , glycoprotein , sialic acid , baculoviridae , virology , microbiology and biotechnology , biology , virus , biochemistry , spodoptera , gene
The gp51‐p30 glycoprotein constituting BLV envelope was expressed in Sf‐21 insect cells by means of recombinant baculoviruses. Post‐infection cell lysates were analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N ‐acetylglucosamine, mannose, galactose and sialic acid were found on recombinant gp51‐p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf‐21 cells during the course of baculovirus infection. The use of different solubilizing reagents was also evaluated in order to rescue recombinant glycoprotein from its subcellular location.