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The extended packaging sequence of MoMLV contains a constitutive mRNA nuclear export function
Author(s) -
King Jason A.,
Bridger Joanna M.,
Gounari Fotini,
Lichter Peter,
Schulz Thomas F.,
Schirrmacher Volker,
Khazaie Khashayarsha
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00948-x
Subject(s) - biology , sequence (biology) , nuclear export signal , open reading frame , mutagenesis , function (biology) , messenger rna , microbiology and biotechnology , virology , gene , peptide sequence , genetics , rna , mutation
The present report shows that incorporation of defined sequences from the Moloney murine leukaemia virus (MoMLV) into Rex dependent expression vectors based on the human T‐cell leukaemia virus (HTLV‐1) allows Rex independent gene expression. Deletion mutagenesis of the MoMLV derived sequences allowed this function to be localised to a 312 nt length sequence overlapping the MoMLV gag p15/p12 open reading frame. This ‘extended packaging sequence’ has been reported to markedly increase the titre of in vitro packaged retroviral vectors. Using fluorescent in situ hybridisation combined with confocal microscopy we show that the 312 nt element can replace Rex mediated nuclear export and expression of transcripts containing HTLV‐1 cis acting repressive elements. Our observations are consistent with the extended packaging sequence of MoMLV exerting a constitutive mRNA nuclear export function.