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Expression of pI Cln in Escherichia coli gives a strong tolerance to hypotonic stress
Author(s) -
Tao Guo-Zhong,
Kobayashi Akira,
Itoh Hideaki,
Tashima Yohtalou
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00946-6
Subject(s) - tonicity , escherichia coli , pi , complementary dna , osmotic shock , biology , microbiology and biotechnology , extracellular , dna , coding region , gene , biochemistry
We amplified the coding region DNA sequence from a rat renal pI Cln cDNA by PCR and expressed the protein in Escherichia coli cells. The cells were exposed to hypotonic conditions followed by spreading them onto LB plates for subsequent colony survival assay. The present study demonstrated that the cells expressing pI Cln exhibit a strong resistance to hypotonic stress. Moreover, the resistance was specifically inhibited by extracellular ATP and some anion channel inhibitors. These findings indicate that the expression of pI Cln directly confers tolerance to hypotonic stress, and pI Cln is concluded to be an important molecule for cell‐volume regulation.