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Operation of glyoxylate cycle in halophilic archaea: presence of malate synthase and isocitrate lyase in Haloferax volcanii
Author(s) -
Serrano Juan Antonio,
Camacho Mónica,
Bonete Maria José
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00911-9
Subject(s) - glyoxylate cycle , haloferax volcanii , isocitrate lyase , malate synthase , halophile , biochemistry , archaea , malate dehydrogenase , enzyme , isocitrate dehydrogenase , lyase , biology , haloarchaea , chemistry , bacteria , genetics , gene
The occurrence of the glyoxylate cycle has not previously been demonstrated in any of the Archaea. In halophilic archaea, only isocitrate lyase activity has been detected. The halophilic archaeon Haloferax volcanii was tested for the presence of the other key enzyme of this pathway, malate synthase. High activities of this enzyme were detected when the carbon source was acetate. Both glyoxylate cycle key enzymes, isocitrate lyase and malate synthase, from Hf . volcanii were purified and characterized.