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Functional characterization of ORCTL2 ‐ an organic cation transporter expressed in the renal proximal tubules
Author(s) -
Reece Mark,
Prawitt Dirk,
Landers John,
Kast Christina,
Gros Philippe,
Housman David,
Zabel Bernhard U.,
Pelletier Jerry
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00907-7
Subject(s) - multidrug resistance associated protein 2 , gene , transport protein , transporter , organic cation transport proteins , biology , microbiology and biotechnology , membrane protein , homology (biology) , chemistry , biochemistry , atp binding cassette transporter , membrane
Chromosome 11p15.5 harbors a gene or genes involved in Beckwith‐Wiedemann syndrome that confer(s) susceptibility to Wilms' tumor, rhabdomyosarcoma, and hepatoblastoma. We have previously identified a transcript at 11p15.5 which encodes a putative membrane transport protein, designated organic cation transporter‐like 2 (ORCTL2), that shares homology with tetracycline resistance proteins and bacterial multidrug resistance proteins. In this report, we have investigated the transport properties of ORCTL2 and show that this protein can confer resistance to chloroquine and quinidine when overexpressed in bacteria. Immunohistochemistry analyses performed with anti‐ORCTL2 polyc.onal antibodies on human renal sections indicate that ORCTL2 is localized on the apical membrane surface of the proximal tubules. These results suggest that ORCTL2 may play a role in the transport of chloroquine and quinidine related compounds in the kidney.