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cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3) 1
Author(s) -
Kiuchi Yuichi,
Suzuki Hiroshi,
Hirohashi Tomoko,
Tyson Charles A,
Sugiyama Yuichi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00899-0
Subject(s) - complementary dna , transporter , multidrug resistance associated protein 2 , organic anion transporter 1 , cloning (programming) , microbiology and biotechnology , biology , excretion , gene expression , phenobarbital , molecular cloning , chemistry , atp binding cassette transporter , gene , biochemistry , endocrinology , computer science , programming language
Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068–1075]. In the present study, we cloned human MRP3 (1527 amino acids) from Caco‐2 cells. Human MRP3 is predominantly expressed in liver, small intestine and colon; hepatic expression of MRP3 was observed in humans but not in normal rats. In HepG2 cells, the expression of MRP3 was induced by phenobarbital. These results suggest that MRP3 may act as an inducible transporter in the biliary and intestinal excretion of organic anions.