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Evidence for a leukotriene A 4 hydrolase in Xenopus laevis skin exudate
Author(s) -
Clamagirand Christine,
Cadel Sandrine,
Barre Nicole,
Cohen Paul
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00885-0
Subject(s) - leukotriene , leukotriene c4 , biochemistry , hydrolase , enzyme , arachidonic acid , leukotriene b4 , xenopus , chemistry , biology , microbiology and biotechnology , inflammation , asthma , immunology , gene
Leukotriene A 4 hydrolase is a cytosolic metalloenzyme of the arachidonic acid biosynthetic pathway responsible for leukotriene A 4 conversion into leukotriene B 4 . In addition to its epoxide hydrolase properties, this enzyme exhibits an aminopeptidase activity which was used as an assay to monitor the purification of a novel form of leukotriene A 4 hydrolase from Xenopus laevis skin exudate. This 70 kDa, secreted, form of leukotriene A 4 hydrolase was identified by immunochemical cross‐reactivity with anti‐human leukotriene A 4 hydrolase antibodies and by its capacity to convert leukotriene A 4 into leukotriene B 4 . Moreover this enzyme produced a second metabolite which could be the leukotriene B 4 isomer 5S,12R‐dihydroxy‐6,10‐trans‐8,14‐cis‐eicosatetraenoic acid, previously shown by Strömberg et al. (Eur. J. Biochem. 238 (1996) 599–605) to be formed by incubation of the leukotriene A 4 with amphibian tissue extracts. Partial amino acid sequencing of peptides generated by endolysin C fragmentation of the purified enzyme confirmed the presence, in X. laevis skin secretions, of a related but distinct form of leukotriene A 4 hydrolase which is likely to be responsible for the production of these eicosanoid metabolites of leukotriene A 4 .