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Intracellular localization and release of eotaxin from normal eosinophils
Author(s) -
Nakajima Toshiharu,
Yamada Hirokazu,
Iikura Motoyasu,
Miyamasu Misato,
Izumi Shinyu,
Shida Hisato,
Ohta Ken,
Imai Toshio,
Yoshie Osamu,
Mochizuki Mitsuru,
Schröder Jens-M,
Morita Yutaka,
Yamamoto Kazuhiko,
Hirai Koichi
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00863-1
Subject(s) - eotaxin , ionomycin , monoclonal antibody , eosinophil , chemokine , immunology , intracellular , chemistry , microbiology and biotechnology , inflammation , biology , antibody , asthma
Eotaxin is a potent and selective CC chemokine for eosinophils and basophils. We established several monoclonal antibodies (Mabs) allowing the neutralization and measurement of human eotaxin. Using the Mabs as probes, we demonstrated that normal eosinophils contained intracellular granule‐associated eotaxin. Quantification of cell‐associated eotaxin in different leukocyte subsets revealed that it was principally expressed in eosinophils. Finally, we showed that normal eosinophils released eotaxin upon stimulation with either of two secretagogues, C5a or ionomycin. These findings raise the possibility that eosinophil‐derived eotaxin contributes to the local accumulation of eosinophils at the site of inflammation.