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Incorporation of monoclonal antibodies in living rat pheochromocytoma PC12 cells
Author(s) -
Alexandrova N.A,
Sveshnikov P.G,
Nagradova N.K,
Grozdova I.D
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00861-8
Subject(s) - monoclonal antibody , pheochromocytoma , antibody , chemistry , microbiology and biotechnology , biology , immunology , endocrinology
PC12 cells permeabilized with a low concentration of digitonin (5 μM) under controlled conditions were loaded with monoclonal antibodies (MoAb) against the regulatory subunit type II (RII) of cAMP‐dependent protein kinase. After digitonin removal from the nutrient medium (DMEM) the loaded cells repaired within 20–30 min and recontinued growth. The inserted MoAb stayed in the repaired cells at least for several hours. MoAb inhibiting the cAMP binding activity of neural RII [Grozdova et al. (1992) Biochem. Int. 27, 811–822; Sveshnikova et al. (1996) Biochem. Int. 39, 1063–1070] were shown to bind the target antigen inside the cells and influence the properties of intracellular protein kinases.