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The effect of chemical oxidation on the fluorescence of the LH1 (B880) complex from the purple bacterium Rhodobium marinum
Author(s) -
Law Christopher J.,
Cogdell Richard J.
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00826-6
Subject(s) - potassium ferricyanide , fluorescence , ferricyanide , chemistry , bacteriochlorophyll , absorption (acoustics) , photochemistry , nuclear chemistry , inorganic chemistry , photosynthesis , biochemistry , materials science , physics , quantum mechanics , composite material
The effect of chemical oxidation on the absorption and fluorescence emission spectra of the LH1 complex from Rhodobium marinum was investigated. Mild chemical oxidation of the LH1 complex, by addition of 10 mM potassium ferricyanide, caused a 2–3% bleaching of the 880‐nm Q y absorption band. In contrast, at the same ferricyanide concentration, fluorescence emission intensity of the LH1 complex was quenched by about 50%. This result demonstrates that oxidation of very few bacteriochlorophyll (BChl) molecules in the LH1 ring is enough to completely quench its fluorescence.