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Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography
Author(s) -
Rogl H,
Kosemund K,
Kühlbrandt W,
Collinson I
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00825-4
Subject(s) - inclusion bodies , chelation , escherichia coli , chemistry , elution , affinity chromatography , membrane , biochemistry , membrane protein , chromatography , bacterial outer membrane , enzyme , organic chemistry , gene
Two distinctly different membrane proteins, which produced inclusion bodies in Escherichia coli , have been refolded to reconstitute properties appropriate to their native counterparts. The method employed utilises nickel chelating chromatography, where the solubilised inclusion bodies bind, refold and elute. Our aims were to release a large pool of membrane protein for functional, mutational and crystallisation screening studies. It is hoped that the methods described here will have a general application for other membrane proteins which have formed inclusion bodies.