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Identification and characterisation of a novel splice variant of synaptojanin1
Author(s) -
Woscholski Rüdiger,
Finan Peter M,
Radley Elisabeth,
Parker Peter J
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00820-5
Subject(s) - splice , phosphatase , alternative splicing , identification (biology) , domain (mathematical analysis) , function (biology) , biology , computational biology , microbiology and biotechnology , chemistry , biochemistry , enzyme , gene , exon , mathematical analysis , botany , mathematics
Synaptojanin1, the major constitutively active PtdInsP 3 5‐phosphatase activity in rat brain, is one of two closely related proteins both extensively spliced in their C‐terminal proline rich domain. We describe here the discovery of a novel splice variant of synaptojanin1 which misses the major N‐terminal part of the SAC1 domain. This ΔSAC‐synaptojanin1 is expressed in rat brain tissue as shown by Northern and Western analysis. However, the deletion of the SAC1 domain does not alter PtdInsP 3 5‐phosphatase activity demonstrating that the SAC1 domain is not necessary for catalytic function.