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Glycerol diversifies phage repertoire selections and lowers non‐specific phage absorption
Author(s) -
Kjær Svend,
Stausbøl-Grøn Brian,
Wind Troels,
Ravn Peter,
Hobolt Jensen Kristian,
Kahns Liselotte,
Clark Brian F.C
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00789-3
Subject(s) - glycerol , phage display , antibody , biology , biochemistry , chemistry , microbiology and biotechnology , genetics
Using a semi‐synthetic phage displayed antibody repertoire, isoform‐specific and cross‐reactive phage‐antibodies to eukaryotic elongation factor 1A (eEF1A) have been selected. Enrichment of specific antibodies was found to depend on the presence of glycerol. Further selections against lactate dehydrogenase (LDH) revealed that the dominance of a phage‐antibody clone to LDH was inhibited by glycerol, a notable feature for selection strategies where a broad variety of binding clones is desired. The impact of glycerol in distinct steps of the selection protocol was examined and glycerol found to affect certain antibody‐antigen interactions. Furthermore, the non‐specific phage binding was lowered by three orders of magnitude at a 20% (v/v) glycerol concentration.