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Mitochondrial formation of hydrogen peroxide is causally linked to the antimycin A‐mediated prevention of tert ‐butylhydroperoxide‐induced U937 cell death
Author(s) -
Brambilla Liliana,
Cantoni Orazio
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00764-9
Subject(s) - hydrogen peroxide , chemistry , antimycin a , cytoprotection , mitochondrion , superoxide , photochemistry , reactive oxygen species , biophysics , microbiology and biotechnology , biochemistry , apoptosis , biology , enzyme
Antimycin A and 2‐heptyl‐4‐hydroxyquinoline N‐oxide (HQNO), both of which bind to the same site of complex III, prevented U937 cell killing promoted by tert ‐butylhydroperoxide (tB‐OOH). This cytoprotection was not directly caused by inhibition of electron transport or reduced formation of tB‐OOH‐derived toxic species, but rather appeared to be the consequence of a mechanism involving mitochondrial formation of hydrogen peroxide. Ubisemiquinone was most likely the electron donor allowing the formation of superoxides and, as a consequence, of hydrogen peroxide.