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Transport of apically but not basolaterally internalized ricin to the Golgi apparatus is stimulated by 8‐Br‐cAMP in MDCK cells
Author(s) -
Llorente Alicia,
van Deurs Bo,
Sandvig Kirsten
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00754-6
Subject(s) - ricin , golgi apparatus , endocytosis , endosome , microbiology and biotechnology , transport protein , chemistry , biology , biochemistry , toxin , cell , endoplasmic reticulum , intracellular
The plant toxin ricin has to be transported to the Golgi apparatus after endocytosis to exert its toxic effect. In this study we show that transport of apically endocytosed ricin to the Golgi apparatus is stimulated by 8‐Br‐cAMP in polarized MDCK cells. This stimulation is counteracted by the PKA inhibitor H‐89. In contrast, there is no increase in the transport to the Golgi apparatus of ricin internalized from the basolateral membrane. These results suggest that protein kinase A selectively regulates endosome to Golgi transport in these cells.