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Re‐expression of C/EBPα induces CYP2B6, CYP2C9 and CYP2D6 genes in HepG2 cells
Author(s) -
Jover Ramiro,
Bort Roque,
Gómez-Lechón M.José,
Castell José V
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00746-7
Subject(s) - cyp2b6 , transcription factor , transfection , cytochrome p450 , microbiology and biotechnology , biology , hepatocyte nuclear factors , transcription (linguistics) , metallothionein , gene , expression vector , promoter , gene expression , alpha (finance) , enzyme , chemistry , recombinant dna , biochemistry , cyp1a2 , medicine , linguistics , philosophy , construct validity , nursing , patient satisfaction
Cytochrome P450 (CYP) activity is very low or even absent in human hepatomas, a phenomenon that is accompanied by low levels of some liver transcription factors, notably C/EBPα. To investigate a possible link between this transcription factor and hepatic CYP expression, we have stably transfected HepG2 cells with a C/EBPα vector containing a Zn‐inducible metallothionein promoter. Expression of functional C/EBPα up to liver levels concomitantly increased the mRNAs of several members of the CYP2 family (2B6, 2C9 and 2D6), suggesting that this transcription factor may play a relevant role in controlling the hepatic expression of CYP enzymes.