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Involvement of endotoxins or tumor necrosis factor‐α in macrophage‐mediated oxidation of low density lipoprotein
Author(s) -
Fujiwara Kyoko,
Sato Hideyo,
Bannai Shiro
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00735-2
Subject(s) - tumor necrosis factor alpha , lipopolysaccharide , macrophage , chemistry , low density lipoprotein , lipoprotein , in vitro , tumor necrosis factor α , biochemistry , microbiology and biotechnology , cholesterol , endocrinology , biology
Oxidation of low‐density lipoprotein (LDL) can be mediated in vitro by cultured cells, including macrophages. This cellular oxidation is dependent on the production of free thiols by the cells in the presence of transition metal ions. We now report that the production of thiols by macrophages is greatly enhanced when cells are cultured with lipopolysaccharide (LPS) or tumor necrosis factor‐α (TNF‐α). Oxidation of LDL by macrophages is markedly augmented by pre‐treatment of the cells with LPS or TNF‐α. The results suggest that activation by endotoxins or TNF‐α is a necessary step for macrophages to mediate oxidation of LDL.