Premium
Antioxidant enzymatic activities and lipid peroxidation in cultured human chondrocytes from vertebral plate cartilage
Author(s) -
Grazioli Vittorio,
Schiavo Roberta,
Casari Erminia,
Marzatico Fulvio,
Rodriguez y Baena Riccardo,
Gaetani Paolo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00731-5
Subject(s) - lipid peroxidation , superoxide dismutase , catalase , glutathione peroxidase , chemistry , in vivo , malondialdehyde , antioxidant , biochemistry , in vitro , cartilage , enzyme , biology , anatomy , microbiology and biotechnology
Total SOD activity increased, even if not significantly, between the 12th and the 18th day. A significant variation of GSHPx ( P <0.01) and of catalase ( P <0.001) activity was observed between the 3rd and the 6th day with no further variation until the 18th day. A significant increase ( P <0.001) of lipid peroxidation from the 3rd to the 18th day was also observed. These results seem to indicate that only fresh human cultured chondrocytes are suitable to study, through in vitro models, the in vivo behavior of the antioxidative status of these cells.