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Altered expression of the DNA repair protein, N ‐methylpurine‐DNA glycosylase (MPG), in breast cancer
Author(s) -
Cerda Sonia R,
Turk Patrick W,
Thor Ann D,
Weitzman Sigmund A
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00697-8
Subject(s) - carcinogenesis , blot , dna glycosylase , microbiology and biotechnology , biology , southern blot , western blot , cancer research , breast cancer , dna repair , dna , dna damage , immunohistochemistry , northern blot , gene , cancer , gene expression , genetics , immunology
We examined expression of N ‐methylpurine‐DNA glycosylase (MPG), a DNA repair enzyme that removes N ‐alkylpurine damage, in normal, malignant, and immortalized breast epithelial cells, and breast cancer cell lines (MDA‐MB‐231, MCF7, T47D). Northern analysis showed increased expression in cancer versus normal breast epithelial cells (2–24‐fold). Southern blots revealed no gene amplification or polymorphisms. Immunofluorescence, immunohistochemistry, and Western blot analysis demonstrated increased MPG protein expression in the tumor cells that correlated with elevated glycosylase activity. Since MPG overexpression has been shown to be paradoxically associated with increased susceptibility to DNA damage, up‐regulation of this gene may suggest a functional role in breast carcinogenesis.