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The antioxidant action of 2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐α]pyrazin‐3‐one (MCLA), a chemiluminescence probe to detect superoxide anions
Author(s) -
Tampo Yoshiko,
Tsukamoto Maki,
Yonaha Masanori
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00689-9
Subject(s) - chemistry , chemiluminescence , antioxidant , lipid peroxidation , superoxide , cumene hydroperoxide , thiobarbituric acid , radical , liposome , biochemistry , oxygen , pharmacology , photochemistry , chromatography , organic chemistry , medicine , enzyme , catalysis
The antioxidant effect of 2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo[1,2‐α]pyrazin‐3‐one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O ⋅− 2 , was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2′‐azobis (2‐amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. MCLA profoundly inhibited the propagation reaction in Fe 2+ ‐dependent lipid peroxidation in liposomes, and MCLA disappearance accompanied by suppression of oxygen consumption markedly occurred in liposomes susceptible to peroxidation. Thiobarbituric acid‐reactive substances in all systems used were also suppressed by MCLA dose dependently. These results indicate that MCLA has an antioxidant property through scavenging free radicals.