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Translation of tobacco chloroplast rps14 mRNA depends on a Shine‐Dalgarno‐like sequence in the 5′‐untranslated region but not on internal RNA editing in the coding region
Author(s) -
Hirose Tetsuro,
Kusumegi Takahiro,
Sugiura Masahiro
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00673-5
Subject(s) - messenger rna , translation (biology) , coding region , untranslated region , eukaryotic translation , rna , biology , protein biosynthesis , five prime untranslated region , chloroplast , shine dalgarno sequence , rna editing , microbiology and biotechnology , genetics , gene
The role of Shine‐Dalgarno‐like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine‐Dalgarno‐like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in the protein‐coding region of the rps14 mRNA. Editing of the second site was found to be partial and hence the partially edited transcripts are accumulated in tobacco green leaves. In vitro translation assays using the fully edited, partially edited and unedited rps14 mRNAs indicated that editing does not directly influence translational efficiency.