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Functional analyses of the Dof domain, a zinc finger DNA‐binding domain, in a pumpkin DNA‐binding protein AOBP
Author(s) -
Shimofurutani Naoko,
Kisu Yasutomo,
Suzuki Masashi,
Esaka Muneharu
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00670-x
Subject(s) - zinc finger , ring finger domain , binding domain , dna binding domain , lim domain , hmg box , dna , dna binding protein , binding site , biology , biochemistry , microbiology and biotechnology , chemistry , transcription factor , gene
AOBP, a DNA‐binding protein in pumpkin, contains a Dof domain that is composed of 52 amino acid residues and is highly conserved in several DNA‐binding proteins of higher plants. The Dof domain has a significant resemblance to Cys2/Cys2 zinc finger DNA‐binding domains of steroid hormone receptors and GATA1, but has a longer putative loop where an extra Cys residue is conserved. We show that the Dof domain in AOBP functions as a zinc finger DNA‐binding domain and suggest that the Cys residue uniquely conserved in the putative loop might negatively regulate the binding to DNA.