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Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state
Author(s) -
Tóth György,
Schlammadinger József,
Sipka Sándor,
Kiss Attila,
Szegedi Gyula,
Rák Kálmán,
Szabó Gábor
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00663-2
Subject(s) - oligonucleotide , k562 cells , peripheral blood mononuclear cell , microbiology and biotechnology , promyelocyte , chemistry , myeloid , biology , cell , dna , biochemistry , immunology , in vitro
The single stranded [ 3 H]oligonucleotide uptake by HL‐60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20–45‐fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6–13×) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL‐60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10–15×). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11×) by DUSF if the cells were subjected to blast transformation.