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Guanine nucleotides protect against kainate toxicity in an ex vivo chick retinal preparation
Author(s) -
Burgos Javier S,
Barat Ana,
Souza Diogo O,
Ramı́rez Galo
Publication year - 1998
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(98)00651-6
Subject(s) - kainate receptor , dnqx , ex vivo , excitotoxicity , kainic acid , nucleotide , pharmacology , chemistry , glutamate receptor , biochemistry , guanine , retinal , in vivo , biology , receptor , in vitro , ampa receptor , genetics , gene
Ex vivo preparations of chick neural retina have been successfully used in the assessment of excitotoxicity and in the evaluation of the protective effects of glutamate antagonists. Using a variation of this approach, and measuring the acute and delayed toxic effects of kainate (KA) in terms of lactate dehydrogenase release, we have shown that guanine nucleotides behave as effective neuroprotecting agents. The anti‐excitotoxic potency of guanine nucleotides (in the case of GMP and GDPβS it is about 100 times lower than that of DNQX, a powerful kainate antagonist) correlates well with their ability to displace KA from retinal KA receptors.